The effect of transmitter antagonists on phasic respiratory neurons.

The activity of inspiratory neurons in the nucleus tractus solitarius (NTS) and of expiratory neurons in the nucleus retroambigualis (RNA) was recorded during the iontophoresis of L-glutamate, bicuculline, and strychnine in chloralose-urethane-anesthetized cat. Bicuculline methoxide caused increased activity of NTS and NRA neurons during their active or burst phases. Neither bicuculline nor strychnine caused increased activity during the silent phases of NTS or NRA neurons. When neurons were driven to fire during their silent phase by L-glutamate, the application of bicuculline methoxide caused a diminution of the evoked activity. It is concluded that both inspiratory and expiratory neurons receive inhibitory inputs during both their active and silent phases. Gamma-aminobutyric acid appears to be gating both the excitatory and inhibitory information to NTS and NRA neurons. Strychnine was generally ineffective in changing the firing patterns of inspiratory and expiratory neurons suggesting a limited role of glycine, taurine, and L-alanine in the respiratory network.