Localization of vasoactive intestinal polypeptide- and avian pancreatic polypeptide-like immunoreactivity in the Golgi apparatus of peripheral neurons.

Using the indirect immunofluorescence technique of Coons and collaborators, vasoactive intestinal polypeptide (VIP)-like immunoreactivity (VIP-LI) and avian pancreatic polypeptide (APP)-like immunoreactivity (APP-LI) was observed in certain neurons of the peripheral nervous system of the cat. In the cell bodies in the cat sympathetic ganglia a strong to very strong VIP-LI or APP-LI was observed with a distribution resembling that of the Golgi apparatus. In addition, a weaker immunoreactivity was seen diffusely in the cytoplasm. After photography, the sections processed for immunohistochemistry were stained with the thiamine pyrophosphatase technique of Novikoff and Goldfischer. The latter technique is assumed to be a specific marker for the Golgi complex. In all cases it was found that the strong peptide immunoreactivity and the thiamine pyrophosphatase activity had an identical distribution. Thus, one pool of these peptides appears to be localized to the Golgi apparatus. Yet another pool is localized to other components, such as vesicles.