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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Hewitt S, Nicholas T E

Comp Biochem Physiol A Comp Physiol. 1983;74(2):467-9. doi: 10.1016/0300-9629(83)90633-3.

Using the isolated perfused lung preparation we have demonstrated a low-activity ureolytic enzyme present in rodent lung tissue. The enzyme shares four characteristic features with jack bean urease (EC 3.5.1.5). 2. Ureolytic activity was inhibited by fluoride ions and methionine hydroxamic acid; using the latter inhibitor, the I50 value and maximum inhibition were similar to those reported for jack bean urease. The apparent Km for rat lung urease was similar to the plasma urea level. 3. The low level of urease activity in the rat lung and in that of Notomys alexis, a desert rodent, suggests that the enzyme is not involved in urea excretion, rather that pulmonary ammonia production may influence fluid balance at the alveolus.

使用离体灌流肺制备方法，我们已证明啮齿动物肺组织中存在一种低活性的尿素分解酶。该酶与刀豆脲酶（EC 3.5.1.5）具有四个共同特征。2. 尿素分解活性受到氟离子和甲硫氨酸羟肟酸的抑制；使用后一种抑制剂时，其I50值和最大抑制程度与报道的刀豆脲酶相似。大鼠肺脲酶的表观Km与血浆尿素水平相似。3. 大鼠肺以及荒漠啮齿动物亚历克西斯荒漠鼠（Notomys alexis）肺中的脲酶活性水平较低，这表明该酶不参与尿素排泄，而是肺部氨的产生可能会影响肺泡处的液体平衡。

Hewitt S, Nicholas T E

Comp Biochem Physiol A Comp Physiol. 1983;74(2):467-9. doi: 10.1016/0300-9629(83)90633-3.

Using the isolated perfused lung preparation we have demonstrated a low-activity ureolytic enzyme present in rodent lung tissue. The enzyme shares four characteristic features with jack bean urease (EC 3.5.1.5). 2. Ureolytic activity was inhibited by fluoride ions and methionine hydroxamic acid; using the latter inhibitor, the I50 value and maximum inhibition were similar to those reported for jack bean urease. The apparent Km for rat lung urease was similar to the plasma urea level. 3. The low level of urease activity in the rat lung and in that of Notomys alexis, a desert rodent, suggests that the enzyme is not involved in urea excretion, rather that pulmonary ammonia production may influence fluid balance at the alveolus.

使用离体灌流肺制备方法，我们已证明啮齿动物肺组织中存在一种低活性的尿素分解酶。该酶与刀豆脲酶（EC 3.5.1.5）具有四个共同特征。2. 尿素分解活性受到氟离子和甲硫氨酸羟肟酸的抑制；使用后一种抑制剂时，其I50值和最大抑制程度与报道的刀豆脲酶相似。大鼠肺脲酶的表观Km与血浆尿素水平相似。3. 大鼠肺以及荒漠啮齿动物亚历克西斯荒漠鼠（Notomys alexis）肺中的脲酶活性水平较低，这表明该酶不参与尿素排泄，而是肺部氨的产生可能会影响肺泡处的液体平衡。