Schneider G B, Byrnes J E
Exp Cell Biol. 1983;51(1):44-50. doi: 10.1159/000163172.
Osteopetrosis in the ia/ia rat is known to be the result of reduced bone resorption due to abnormal osteoclasts. Studies in this mutant have shown that mononuclear cells from normal littermates could cure the skeletal sclerosis and result in the formation of normal osteoclasts when transplanted into ia/ia rats. This investigation was pursued in an attempt to determine the cellular source of this cure by transplanting various populations of cells from 21-day-old normal rats to unrelated newborn ia/ia recipients. The effects of treatment were evaluated radiographically and by measuring the size of the tibial marrow cavity. The cellular suspensions that were effective in curing the disease were the Ficoll-Hypaque isolate of spleen, bone marrow, and newborn livers. The Ficoll-Hypaque isolates of lymph node, thymus, and blood, and the adherent pool of peritoneal cells and splenic cells did not produce a cure in the ia/ia recipients. These results suggest that the cellular source of the cure is a stem cell. This conclusion is further substantiated by the finding that Thy 1.1 antigen (a stem cell marker in the rat) is expressed on a majority of the cells from the donor sources that affected a cure.
已知ia/ia大鼠的骨硬化症是由于破骨细胞异常导致骨吸收减少的结果。对这种突变体的研究表明，来自正常同窝仔鼠的单核细胞在移植到ia/ia大鼠体内时，可以治愈骨骼硬化并导致正常破骨细胞的形成。本研究旨在通过将21日龄正常大鼠的各种细胞群移植到无关的新生ia/ia受体中，来确定这种治愈方法的细胞来源。通过放射学检查和测量胫骨髓腔大小来评估治疗效果。有效治愈该疾病的细胞悬液是脾脏、骨髓和新生肝脏的Ficoll-Hypaque分离物。淋巴结、胸腺和血液的Ficoll-Hypaque分离物，以及腹膜细胞和脾细胞的贴壁细胞群，在ia/ia受体中均未产生治愈效果。这些结果表明，治愈的细胞来源是一种干细胞。大鼠Thy 1.1抗原（一种干细胞标志物）在大多数来自能产生治愈效果的供体来源的细胞上表达，这一发现进一步证实了这一结论。
