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Ly-6缺陷的遗传基础：Ly-6缺陷型和Thy-1缺陷型突变细胞系之间的互补作用。

Genetic basis for Ly-6- defect: complementation between Ly-6- and Thy-1- mutant cell lines.

作者信息

Horton M A, Hyman R

出版信息

Immunogenetics. 1983;17(3):261-70. doi: 10.1007/BF00364410.

DOI:10.1007/BF00364410
PMID:6131864
Abstract

Selection of antigen loss variants from the BW5147 cell line after chemical mutagenesis, using antibodies to Thy-1.1, Ly-6.2 and H9/25 allospecificities, produced cell lines with a pleiotropic defect in cell surface antigen expression; selection against any antigen led to the loss of all three determinants. The genetic basis for the mutant phenotype has been analyzed by gene complementation by somatic cell hybridization between Ly-6- or H9/25- and Thy-1- mutants, which comprise different Thy-1 gene complementation classes. The Ly-6- and H9/25- mutants can be classified as belonging to either the A or E Thy-1- mutant class.

摘要

在化学诱变后，利用针对Thy-1.1、Ly-6.2和H9/25同种特异性的抗体从BW5147细胞系中筛选抗原缺失变体，得到了细胞表面抗原表达存在多效性缺陷的细胞系；针对任何一种抗原进行筛选都会导致所有这三种决定簇的缺失。通过Ly-6或H9/25突变体与Thy-1突变体之间的体细胞杂交进行基因互补分析了突变表型的遗传基础，这些突变体属于不同的Thy-1基因互补类。Ly-6和H9/25突变体可归类为属于A类或E类Thy-1突变体。

相似文献

1
Genetic basis for Ly-6- defect: complementation between Ly-6- and Thy-1- mutant cell lines.Ly-6缺陷的遗传基础：Ly-6缺陷型和Thy-1缺陷型突变细胞系之间的互补作用。
Immunogenetics. 1983;17(3):261-70. doi: 10.1007/BF00364410.
2
Further analysis of the Ly-6 genetic region: isolation and characterization of cell lines with altered expression of Ly-6 antigens.
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Sodium butyrate causes reexpression of three membrane proteins on glycolipid-anchoring mutants.丁酸钠可使糖脂锚定突变体上的三种膜蛋白重新表达。
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4
Genetic linkage of Ly-6 and Thy-1 loci in the mouse.小鼠中Ly-6和Thy-1基因座的遗传连锁
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Changes in expression of Ia, Thy-1, and Ly-5 antigens in epidermal cells during delayed contact sensitivity reactions in mice: flow cytometric analysis.小鼠迟发型接触性敏感反应期间表皮细胞中Ia、Thy-1和Ly-5抗原表达的变化：流式细胞术分析
J Invest Dermatol. 1986 Feb;86(2):121-4. doi: 10.1111/1523-1747.ep12284113.
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No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes.在四种不同互补类别的Thy-1阴性突变型胸腺瘤细胞中，没有糖脂锚定物添加到Thy-1糖蛋白上。
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引用本文的文献

1
Mutants of cultured mouse cells deficient in Ly-2 antigen.缺乏Ly-2抗原的培养小鼠细胞突变体。
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2
Mutations that influence the secretory path in animal cells.影响动物细胞分泌途径的突变。
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3
The 33,000 protein precipitated by Ly-6A.2-specific antibodies is not associated with the Ly-6 polymorphism.由Ly-6A.2特异性抗体沉淀的33000种蛋白质与Ly-6多态性无关。

本文引用的文献

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Expression of Ly-6 on activated T and B cells: Possible identity with Ala-1.Ly-6 在活化 T 和 B 细胞上的表达：可能与其 Ala-1 具有同一性。
Immunogenetics. 1978 Dec;7(1):537-43. doi: 10.1007/BF01844042.
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Serological properties of anti-Ly-6.2 serum produced by a new immunization schedule.通过新免疫方案产生的抗Ly-6.2血清的血清学特性。
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H 9/25 monoclonal antibody recognizes a new allospecificity of mouse lymphocyte subpopulations: strain and tissue distribution.
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Isolation of a murine Ly-6 cDNA reveals a new multigene family.一种小鼠Ly-6 cDNA的分离揭示了一个新的多基因家族。
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Biochemistry of the glycosyl-phosphatidylinositol membrane protein anchors.糖基磷脂酰肌醇膜蛋白锚定物的生物化学
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No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes.在四种不同互补类别的Thy-1阴性突变型胸腺瘤细胞中，没有糖脂锚定物添加到Thy-1糖蛋白上。
Mol Cell Biol. 1988 Feb;8(2):674-8. doi: 10.1128/mcb.8.2.674-678.1988.
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Murine Ly-6 multigene family is located on chromosome 15.小鼠Ly-6多基因家族位于15号染色体上。
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Cell-surface-antigen mutants of haematopoietic cells. Tools to study differentiation, biosynthesis and function.造血细胞的细胞表面抗原突变体。用于研究分化、生物合成和功能的工具。
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9
Two different mutants blocked in synthesis of dolichol-phosphoryl-mannose do not add glycophospholipid anchors to membrane proteins: quantitative correction of the phenotype of a CHO cell mutant with tunicamycin.两种在多萜醇磷酸甘露糖合成中受阻的不同突变体不会将糖磷脂锚添加到膜蛋白上：用衣霉素对CHO细胞突变体表型的定量校正。
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H 9/25单克隆抗体识别小鼠淋巴细胞亚群的一种新的同种特异性：品系和组织分布。
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4
The primary glycosylation defect in class E Thy-1-negative mutant mouse lymphoma cells is an inability to synthesize dolichol-P-mannose.E类Thy-1阴性突变小鼠淋巴瘤细胞中的主要糖基化缺陷是无法合成多萜醇磷酸甘露糖。
J Biol Chem. 1980 May 25;255(10):4441-6.
5
Ly-6 region regulates expression of multiple allospecificities.Ly-6区域调节多种同种特异性的表达。
Immunogenetics. 1981;13(5):435-41. doi: 10.1007/BF00346024.
6
Molecular characterization of the Ly-6.2 antigen.Ly-6.2抗原的分子特征
Cell Immunol. 1981 Oct;64(1):187-91. doi: 10.1016/0008-8749(81)90469-x.
7
Effect of gene dosage on cell-surface expression of Thy-1 antigen in somatic cell hybrids between Thy-1- Abelson-leukemia-virus induced lymphomas and Thy-1+ mouse lymphomas.基因剂量对Thy-1 - 阿贝尔森白血病病毒诱导的淋巴瘤与Thy-1 + 小鼠淋巴瘤之间体细胞杂交瘤中Thy-1抗原细胞表面表达的影响。
Immunogenetics. 1981;12(3-4):381-95. doi: 10.1007/BF01561678.
8
Production of monoclonal antibodies against MM antigen: the serologic identification of MM antigen with Ly-6.2 alloantigen.抗骨髓瘤（MM）抗原单克隆抗体的制备：利用Ly-6.2同种异体抗原对MM抗原进行血清学鉴定。
J Immunol. 1982 Jan;128(1):201-5.
9
Studies of the mouse Ly-6 alloantigen system. I. Serological characterization of mouse Ly-6 alloantigen by monoclonal antibodies.小鼠Ly-6同种异体抗原系统的研究。I. 用单克隆抗体对小鼠Ly-6同种异体抗原进行血清学鉴定。
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10
Monoclonal antibodies to ThB detect close linkage of Ly-6 and a gene regulating ThB expression.针对ThB的单克隆抗体检测到Ly-6与一个调节ThB表达的基因紧密连锁。
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