Mondorf A W, Heynold F T, Scherberich J E, Hess H, Schoeppe W
Infection. 1983;11 Suppl 1:S57-62. doi: 10.1007/BF01641109.
Ten informed healthy volunteers with normal renal function received 2 X 3.0 g ceftazidime intravenously for three consecutive days. Four weeks later, ceftazidime was combined with 1 X 3 mg/kg body weight tobramycin, administered intramuscularly, for three consecutive days. The effect of the two treatments on parameters used to assess renal function was examined prior to administration, during administration for three days and for a follow-up period. Alanine-aminopeptidase (AAP) levels in 24 h urine samples were measured in addition to kidney function parameters. The urine from the volunteer who had shown the highest AAP levels in each series was examined by ultracentrifugation for the presence of membrane particles with AAP activity. Ceftazidime showed no effect on the proximal tubular membrane. No increased elimination of AAP could be demonstrated. The kidney function parameters remained unchanged. The combination of ceftazidime with tobramycin led to a cumulative increase in AAP activity which was not significantly different from the increase observed when the same dose of tobramycin is administered alone. No additive effects could be demonstrated. Ultracentrifugation showed no indication of an impairment of the membrane integrity when ceftazidime was administered alone or in combination with tobramycin.
十名肾功能正常的知情健康志愿者连续三天静脉注射2×3.0克头孢他啶。四周后，头孢他啶与1×3毫克/千克体重的妥布霉素联合，连续三天肌肉注射。在给药前、给药三天期间及随访期，检查了这两种治疗对用于评估肾功能的参数的影响。除肾功能参数外，还测量了24小时尿液样本中的丙氨酸氨基肽酶（AAP）水平。通过超速离心检查每个系列中AAP水平最高的志愿者的尿液中是否存在具有AAP活性的膜颗粒。头孢他啶对近端肾小管膜无影响。未证明AAP的消除增加。肾功能参数保持不变。头孢他啶与妥布霉素联合导致AAP活性累积增加，这与单独给予相同剂量妥布霉素时观察到的增加无显著差异。未证明有相加作用。超速离心显示，单独给予头孢他啶或与妥布霉素联合使用时，均未显示膜完整性受损的迹象。
