Gaspar P, Berger B, Gay M, Hamon M, Cesselin F, Vigny A, Javoy-Agid F, Agid Y
J Neurol Sci. 1983 Feb;58(2):247-67. doi: 10.1016/0022-510x(83)90221-6.
The immunocytochemical localization of tyrosine hydroxylase (TH) and methionine-enkephalin (met-enkephalin) was determined at two representative caudal and rostral levels of the human mesencephalon. Four main groups of catecholaminergic neurons were delineated, situated in the substantia nigra and the lateral, ventromedial and dorsomedial tegmentum, extending over several cytoarchitectonic divisions. They matched fairly well the dopaminergic cell groups described in monkey midbrain. TH-like immunoreactivity and neuromelanin were closely related in neurons of substantia nigra, but less so in the other groups. A widespread met-enkephalinergic innervation was observed in most areas containing catecholaminergic neurons. It followed a characteristic pattern: homogeneous and very dense in the lateral and posterior portions of substantia nigra; patchy and less dense in the other areas, the medio-ventral and periaqueductal gray being only sparsely innervated, in contrast to observations in rodents. Dopaminergic cell bodies surrounded by met-enkephalinergic varicosities were seen in most groups, particularly in the lateral substantia nigra and medioventral tegmentum. The topography of met-enkephali-like immunoreactive terminals in the substantia nigra was reminiscent of the distribution of neostriatal and pallidal afferents.
在人类中脑两个具有代表性的尾侧和嘴侧水平，确定了酪氨酸羟化酶（TH）和甲硫氨酸脑啡肽（甲硫脑啡肽）的免疫细胞化学定位。确定了四组主要的儿茶酚胺能神经元，位于黑质以及外侧、腹内侧和背内侧被盖，延伸跨越几个细胞构筑分区。它们与猴中脑描述的多巴胺能细胞群相当吻合。在黑质神经元中，TH样免疫反应性与神经黑素密切相关，但在其他组中相关性较弱。在大多数含有儿茶酚胺能神经元的区域观察到广泛的甲硫脑啡肽能神经支配。它遵循一种特征模式：在黑质的外侧和后部均匀且非常密集；在其他区域呈斑片状且密度较低，与啮齿动物的观察结果相反，中腹侧和导水管周围灰质仅稀疏受支配。在大多数组中，尤其是在黑质外侧和中腹侧被盖，可见被甲硫脑啡肽能曲张体包围的多巴胺能细胞体。黑质中甲硫脑啡肽样免疫反应性终末的拓扑结构让人联想到新纹状体和苍白球传入纤维的分布。
