Puciłowski O, Płaźnik A, Kostowski W
Pol J Pharmacol Pharm. 1982;34(1-3):107-13.
MIF-1 (Pro-Leu-Gly-NH2) is an endogenous substance which seems to be involved in the regulation of some central nervous system functions. It was recently shown to affect learning and memory processes. In order to further clarify the role of MIF in these processes we used two different passive avoidance tests: one-trial step down (SD) and shock suppressed drinking (SSD). Z-Pro-Leu-Gly-NH2 was administered on the training day ip either 15 minutes before trial or immediately after. The doses used were as follows: SD--1.0 mg/kg, SSD--0.05 mg/kg (in the group injected before trial) and 1.0 mg/kg--SD and SSD, in the groups injected post-trial. Only the immediate post-training injection of peptide resulted in significant prolongation of the step-down latencies on the retention-day. Similarly, in the SSD procedure only post-training MIF markedly attenuated water intake on the day after training. In a separate experiment we have found that in the dose of 1.0 mg/kg, but not 0.1 mg/kg, MIF significantly inhibited water intake. These results suggest that this naturally occurring peptide can contribute to the processes of memory consolidation and water intake.
MIF-1（脯氨酸-亮氨酸-甘氨酸-氨基）是一种内源性物质，似乎参与某些中枢神经系统功能的调节。最近研究表明它会影响学习和记忆过程。为了进一步阐明MIF在这些过程中的作用，我们采用了两种不同的被动回避试验：一次性跳台试验（SD）和电击抑制饮水试验（SSD）。在训练当天腹腔注射Z-脯氨酸-亮氨酸-甘氨酸-氨基，要么在试验前15分钟注射，要么在试验后立即注射。使用的剂量如下：在试验前注射的组中，SD试验用1.0毫克/千克，SSD试验用0.05毫克/千克；在试验后注射的组中，SD和SSD试验均用1.0毫克/千克。只有在训练后立即注射该肽才会使在记忆保持日的跳台潜伏期显著延长。同样，在SSD试验中，只有训练后注射MIF才会在训练后第二天显著减少饮水量。在另一项实验中，我们发现剂量为1.0毫克/千克而非0.1毫克/千克时，MIF会显著抑制饮水。这些结果表明，这种天然存在的肽可能有助于记忆巩固和饮水过程。
