Cain H, Kraus B, Krauspe R, Osborn M, Weber K
Virchows Arch B Cell Pathol Incl Mol Pathol. 1983;42(1):65-81. doi: 10.1007/BF02890371.
Comparative immunofluorescence microscopic, transmission and scanning electron microscopic investigations were carried out to study the arrangement and significance of vimentin filaments in monocytes, macrophages, epithelioid cell equivalents and multinucleate giant cells under various different functional conditions, and in the presence of functional disorders. Uncoated or sebum-coated coverslips were implanted in the peritoneal cavity of Wistar rats. Some of the animals received repeated i.p. injections of colchicine. Rats were killed at various times 1 to 14 days after initiation of the experiment. The number of macrophages, the degree of their activation, and the growth of cells on the coverslips was considerably greater on sebum-coated than on uncoated implants. Various characteristic vimentin distribution patterns were found dependent on the cell cycle, the form and volume of the cell, and on the degree of differentiation and maturity; they were also related to the type and intensity of cell function. These patterns were best developed in ordered multinucleate giant cells. Repeated administrations of colchicine resulted in a marked flattening of the cell body on the coverslips--which correlated with a considerable reduction in the number of vimentin filaments and of cytoplasmic processes--and also in the formation of circumscribed erect, tree-like protuberances. The "trunk" of these structures comprised closely bundled vimentin filaments, and the cell nucleus was located at its base. These morphologic changes, which were associated with a functional insufficiency, proved to be reversible.
进行了免疫荧光显微镜、透射电子显微镜和扫描电子显微镜的对比研究，以探讨波形蛋白丝在不同功能状态及功能紊乱情况下，在单核细胞、巨噬细胞、类上皮细胞及多核巨细胞中的排列方式及其意义。将未包被或涂有皮脂的盖玻片植入Wistar大鼠的腹腔。部分动物腹腔内反复注射秋水仙碱。在实验开始后的1至14天内的不同时间点处死大鼠。与未包被的植入物相比，涂有皮脂的植入物上巨噬细胞数量更多，其活化程度更高，盖玻片上细胞生长更为旺盛。根据细胞周期、细胞形态和体积、分化和成熟程度发现了各种特征性波形蛋白分布模式；它们还与细胞功能的类型和强度有关。这些模式在有序的多核巨细胞中表现最为明显。反复给予秋水仙碱导致盖玻片上细胞体明显变平，这与波形蛋白丝数量和细胞质突起数量显著减少相关，还导致形成界限分明的直立树状突起。这些结构的“主干”由紧密束状的波形蛋白丝组成，细胞核位于其基部。这些与功能不足相关的形态学变化被证明是可逆的。
