Azadova N B, Zhdanov V M
Acta Virol. 1982 Dec;26(6):427-31.
Comparative analysis of the ribonucleoprotein RNA synthesis was performed in two persistently infected L cell systems. In the first (LSV5-I), cells were infected with the cloned standard SV5 virus, in the second (LSV5-II), infecting virus had been enriched with defective interfering particles (DIP). The LSV5(I) system in its 40th-42nd passages was similar to LSV5(II) at the 2nd-3rd passage levels. There was shown that the ribonucleoprotein 3H-RNA synthesized falls into two classes: the minor corresponding to 50S viral RNA and the major revealing predominantly low molecular RNA. The decrease of the synthesis of the heavy viral RNA fraction and the prevalence of the low molecular RNA promoted the limitation of infection, the survival of cells and prolonged the carrier state. The possible correlation between low molecular RNA synthesis and DIP formation in the L cell-SV5 system is discussed.
在两个持续感染的L细胞系统中进行了核糖核蛋白RNA合成的比较分析。在第一个系统（LSV5-I）中，细胞用克隆的标准SV5病毒感染；在第二个系统（LSV5-II）中，感染病毒富含缺陷干扰颗粒（DIP）。LSV5（I）系统在其第40至42代时与LSV5（II）系统在第2至3代水平时相似。结果表明，合成的核糖核蛋白3H-RNA分为两类：少量的对应于50S病毒RNA，大量的主要显示低分子RNA。重病毒RNA组分合成的减少和低分子RNA的占优促进了感染的限制、细胞的存活并延长了携带状态。讨论了L细胞-SV5系统中低分子RNA合成与DIP形成之间可能的相关性。
