[Synthesis of RNA-containing virus-specific components in L cells persistently infected with SV5 virus].

An analysis of RNA-containing virus-specific structures was performed in the course of the establishment of persistent infection with SV5 virus in L cells. It was observed that up to the 11th passage in the LSV5 system, the same virus-specific structures were synthesized as in acute infection. At the level of the 18th passage synthesis of intracellular RNP was reduced and high 3H-uridine incorporation into light structures was observed. In the last passages examined (25th and 31st), the structures with rho 1.18--1.17 g/ml were predominant. The sedimentation RNA analysis (the 3rd, 6th, and 11th passages) revealed 50 S, 35--32 S, 24 S, and 18 S RNA. At later passages (25th and 31st), synthesis of 35 S, 24--22 S and lighter RNAs was dominant. The possibility of relationship between reduced 50 S RNA synthesis and low infectivity of intracellular virus is discussed.