Azadova N B, Zhdanov V M
Vopr Virusol. 1980 Sep-Oct(5):558-63.
An analysis of RNA-containing virus-specific structures was performed in the course of the establishment of persistent infection with SV5 virus in L cells. It was observed that up to the 11th passage in the LSV5 system, the same virus-specific structures were synthesized as in acute infection. At the level of the 18th passage synthesis of intracellular RNP was reduced and high 3H-uridine incorporation into light structures was observed. In the last passages examined (25th and 31st), the structures with rho 1.18--1.17 g/ml were predominant. The sedimentation RNA analysis (the 3rd, 6th, and 11th passages) revealed 50 S, 35--32 S, 24 S, and 18 S RNA. At later passages (25th and 31st), synthesis of 35 S, 24--22 S and lighter RNAs was dominant. The possibility of relationship between reduced 50 S RNA synthesis and low infectivity of intracellular virus is discussed.
在L细胞中建立SV5病毒持续感染的过程中，对含RNA病毒特异性结构进行了分析。观察到在LSV5系统传至第11代时，合成的病毒特异性结构与急性感染时相同。在第18代时，细胞内RNP的合成减少，观察到大量3H-尿苷掺入轻结构中。在所检测的最后几代（第25代和第31代）中，密度为1.18--1.17 g/ml的结构占主导。沉降RNA分析（第3代、第6代和第11代）显示有50 S、35--32 S、24 S和18 S RNA。在随后几代（第25代和第31代）中，35 S、24--22 S及更轻的RNA合成占主导。文中讨论了50 S RNA合成减少与细胞内病毒低感染性之间存在关联的可能性。
