Fésüs L, Horváth A, Hársfalvi J
FEBS Lett. 1983 May 2;155(1):1-5. doi: 10.1016/0014-5793(83)80196-3.
A specific interaction between purified liver transglutaminase and small unilamellar phospholipid vesicles at the lipid phase transition have been revealed. The enzyme-induced perturbation of the bilayer is sufficient for phase transition release of encapsulated carboxyfluorescein from the vesicles. The size of the enzyme-phospholipid recombinants depends upon the protein-phospholipid ratio as shown on Sepharose 4B elution profile. The activity of transglutaminase inserted into the bilayer is greatly reduced. The interaction does not occur when the phospholipid vesicle are in the solid or liquid phase and it requires the structural integrity of the enzyme.
已揭示纯化的肝脏转谷氨酰胺酶与处于脂质相变的小单层磷脂囊泡之间存在特定相互作用。酶诱导的双层扰动足以使包裹在囊泡中的羧基荧光素从囊泡中发生相变释放。酶 - 磷脂重组体的大小取决于蛋白质 - 磷脂比例，如在琼脂糖4B洗脱图谱上所示。插入双层中的转谷氨酰胺酶活性大大降低。当磷脂囊泡处于固相或液相时，这种相互作用不会发生，并且它需要酶的结构完整性。
