从大鼠下丘脑富含突触体的部分释放免疫反应性β-内啡肽。

Release of immunoreactive beta-endorphin from synaptosome-enriched fraction of rat hypothalami.

作者信息

Fukata J, Nakai Y, Imura H

出版信息

Neurosci Lett. 1980 Aug;19(1):79-83. doi: 10.1016/0304-3940(80)90259-1.

Abstract

Immunoreactive beta-endorphin is localized mainly in the synaptosome-enriched subcellular fraction from rat hypothalami. In this report, we demonstrate that release of immunoreactive beta-endorphin from the synaptosome-enriched subcellular fraction is increased by 150% in a medium containing high potassium. This stimulatory effect was dependent on temperature and calcium ion in the medium. These results suggest that hypothalamic beta-endorphin can be released from nerve endings during membrane depolarization processes.

摘要

免疫反应性β-内啡肽主要定位于来自大鼠下丘脑的富含突触体的亚细胞部分。在本报告中，我们证明在含有高钾的培养基中，富含突触体的亚细胞部分释放的免疫反应性β-内啡肽增加了150%。这种刺激作用取决于培养基中的温度和钙离子。这些结果表明，下丘脑β-内啡肽可以在膜去极化过程中从神经末梢释放出来。