体外培养大鼠下丘脑β-促脂素和β-内啡肽的释放

Release of beta-lipotropin and beta-endorphin from rat hypothalami in vitro.

作者信息

Vermes I, Mulder G H, Berkenbosch F, Tilders F J

出版信息

Brain Res. 1981 Apr 27;211(1):248-54. doi: 10.1016/0006-8993(81)90092-5.

Abstract

Hypothalamic tissue extracts of rats were chromatographed and beta-endorphin immunoreactivity (beta-Endi) was measured. The two major peaks of beta-Endi co-eluted with beta-lipotropin (beta-LPH) and beta-End respectively. Hypophysectomy caused a local decrease of beta-LPH and beta-End concentrations in the mediobasal hypothalamus. During superfusion of hypothalamic tissue blocks in vitro, membrane depolarization by electric stimulation or 45 mM k+ induced a Ca2+-dependent release of both beta-LPH and beta-End.

摘要

对大鼠下丘脑组织提取物进行色谱分析，并测定β-内啡肽免疫反应性（β-Endi）。β-Endi的两个主要峰分别与β-促脂素（β-LPH）和β-内啡肽（β-End）共洗脱。垂体切除导致下丘脑中间基底部的β-LPH和β-End浓度局部降低。在体外对下丘脑组织块进行灌流时，电刺激或45 mM钾离子引起的膜去极化诱导了β-LPH和β-End的钙离子依赖性释放。