Tuttle J B
Science. 1983 May 27;220(4600):977-9. doi: 10.1126/science.6133352.
Parasympathetic neurons, when cultured alone, lose sensitivity to acetylcholine, but if striated muscle is included in the culture, neuronal chemosensitivity is maintained. The membrane remnants of myotubes ruptured by osmotic shock also supported the responsiveness of the cultured neurons to transmitter, whereas muscle-conditioned medium or membrane remnants of nonmuscle embryonic skin cells did not support this responsiveness. The regulation of chemosensitivity by contact of neurons with the target cell membrane may be important in the formation and maintenance of neuronal circuitry.
单独培养时，副交感神经元会失去对乙酰胆碱的敏感性，但如果培养物中包含横纹肌，神经元的化学敏感性就能得以维持。因渗透压休克而破裂的肌管膜残余物也能维持培养神经元对递质的反应能力，而肌肉条件培养基或非肌肉胚胎皮肤细胞的膜残余物则不能维持这种反应能力。神经元与靶细胞膜接触对化学敏感性的调节，可能在神经回路的形成和维持过程中发挥重要作用。
