Lotzová E, Savary C A, Pollack S B
Transplantation. 1983 May;35(5):490-4. doi: 10.1097/00007890-198305000-00019.
We have studied the effect of in vivo administration of NK 1.1 antiserum on two functions of natural killer (NK) cells: (A) in vitro cytotoxicity of B6 mice to T cell lymphoma, YAC-1, and (B) potential of B6 mice to reject allogeneic BALB/c bone marrow transplants. We demonstrated that a single i.v. injection of 0.4 ml of NK 1.1 antiserum significantly reduced in vitro NK cell cytotoxic potential and concomitantly prevented rejection of allogeneic bone marrow transplants. NK 1.1 antiserum was effective in diminishing both of these functions when injected 2, 6, or 24 hr before transplantation and NK cell assay, but it was ineffective when given 48 hr before transplantation or the NK cell test. As a specificity control, we investigated the effect of specific anti-T-cell-directed monoclonal antibodies, Thy 1.2 and Lyt 2.2, in the same systems. Neither of these antibodies exerted any effect on NK cell cytotoxicity to YAC-1 or rejection of allogeneic bone marrow transplants. These studies indicate that NK cells represent one of the major components of the mechanism of bone marrow graft rejection.
我们研究了体内注射NK 1.1抗血清对自然杀伤（NK）细胞两种功能的影响：（A）B6小鼠对T细胞淋巴瘤YAC-1的体外细胞毒性，以及（B）B6小鼠排斥同种异体BALB/c骨髓移植的能力。我们证明，静脉注射0.4 ml NK 1.1抗血清可显著降低体外NK细胞的细胞毒性潜力，并同时阻止同种异体骨髓移植的排斥反应。在移植和NK细胞检测前2、6或24小时注射NK 1.1抗血清，对这两种功能均有减弱作用，但在移植或NK细胞检测前48小时注射则无效。作为特异性对照，我们在相同系统中研究了特异性抗T细胞导向单克隆抗体Thy 1.2和Lyt 2.2的作用。这些抗体对NK细胞对YAC-1的细胞毒性或同种异体骨髓移植的排斥反应均无任何影响。这些研究表明，NK细胞是骨髓移植排斥机制的主要组成部分之一。
