通过X射线和中子衍射确定重构肌质网膜内单独的Ca2+泵蛋白和磷脂谱结构。

The determination of the separate Ca2+ pump protein and phospholipid profile structures within reconstituted sarcoplasmic reticulum membranes via X-ray and neutron diffraction.

作者信息

Herbette L, Scarpa A, Blasie J K, Wang C T, Hymel L, Seelig J, Fleischer S

出版信息

Biochim Biophys Acta. 1983 May 5;730(2):369-78. doi: 10.1016/0005-2736(83)90354-1.

Abstract

We have previously compared the electron density profiles for several highly-functional reconstituted sarcoplasmic reticulum membranes with that for the isolated sarcoplasmic reticulum membrane (Herbette, L., Scarpa, A., Blasie, J.K., Wang, C.T., Saito, A. and Fleischer, S. (1981) Biophys. J. 36, 47-72). In this paper, we compare the separate calcium pump protein profile within these reconstituted sarcoplasmic reticulum membranes, as derived by X-ray and neutron diffraction methods, with that within isolated sarcoplasmic reticulum membranes. In addition, the time-average perturbation of the lipid bilayer by the incorporated calcium pump protein within these reconstituted sarcoplasmic reticulum membranes has been determined in some detail.

摘要

我们之前已将几种高功能重组肌浆网膜的电子密度分布与分离的肌浆网膜的电子密度分布进行了比较（赫贝特，L.，斯卡帕，A.，布拉西，J.K.，王，C.T.，斋藤，A.和弗莱舍尔，S.（1981年）《生物物理杂志》36卷，47 - 72页）。在本文中，我们将通过X射线和中子衍射方法得出的这些重组肌浆网膜内单独的钙泵蛋白分布与分离的肌浆网膜内的钙泵蛋白分布进行比较。此外，还较为详细地测定了这些重组肌浆网膜内嵌入的钙泵蛋白对脂质双层的时间平均扰动。