使用中子衍射直接测定二棕榈酰卵磷脂多层膜的钙分布结构。
Direct determination of the calcium profile structure for dipalmitoyllecithin multilayers using neutron diffraction.
作者信息
Herbette L, Napolitano C A, McDaniel R V
出版信息
Biophys J. 1984 Dec;46(6):677-85. doi: 10.1016/S0006-3495(84)84066-7.
Abstract
The distribution of calcium in lamellar phases of dipalmitoyllecthin (DPPC) multilayers was directly determined by neutron diffraction and stable isotope substitution of 44Ca for 40Ca. A significant resonance effect on the intensities of the lamellar diffraction pattern was observed for millimolar concentrations of these calcium isotopes. The calcium difference profile indicated that calcium was localized in the phospholipid headgroup region, being excluded from the hydrocarbon core as was water separately determined from the water profile structure obtained by H2O/D2O exchange. A reciprocal space analysis of the difference structure factors indicated that calcium binds preferentially to within 1-2 A of the phosphate moiety of the phospholipid head groups of the DPPC bilayer.
摘要
通过中子衍射以及用(^{44}Ca)替代(^{40}Ca)的稳定同位素取代法，直接测定了二棕榈酰卵磷脂（DPPC）多层膜片层相中钙的分布情况。对于这些钙同位素的毫摩尔浓度，在片层衍射图案的强度上观察到了显著的共振效应。钙差异分布图表明，钙定位于磷脂头部基团区域，如同通过(H_2O/D_2O)交换获得的水分布图结构单独测定的那样，被排除在烃核之外。对差异结构因子的倒易空间分析表明，钙优先结合在DPPC双层磷脂头部基团磷酸部分的1 - 2埃范围内。
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