Ridgway E C, Klibanski A, Martorana M A, Milbury P, Kieffer J D, Chin W W
Endocrinology. 1983 Jun;112(6):1937-42. doi: 10.1210/endo-112-6-1937.
The effects of somatostatin (SRIF) on the production and release of TSH and its subunits have been investigated in bovine anterior pituitary monolayer cultures. SRIF caused a dose-dependent inhibition of TSH and subunit release by TRH, with a half-maximal effective dose of 3 X 10(-8) M. This effect was time dependent and was greater for TSH than for its subunits. However, the basal release and total production of TSH and its subunits over a 24-h period were not affected by SRIF. The effect of SRIF was additive to that of thyroid hormones in suppressing the release of TSH and its subunits by TRH. A combination of SRIF and thyroid hormone completely suppressed the release of TSH and its subunits by TRH. In contrast, thyroid hormones caused a dose-dependent inhibition of the total production, as well as the release, of TSH and its subunits induced by TRH. Furthermore, thyroid hormones produced a dose-dependent increase (r = 0.81; P less than 0.001) in the effectiveness of a single dose of SRIF in suppressing TSH release by TRH. Analysis of these data revealed that thyroid hormones interacted synergistically with the SRIF effect to suppress TRH-mediated TSH and subunit release.
在牛垂体前叶单层培养物中，研究了生长抑素（SRIF）对促甲状腺激素（TSH）及其亚基产生和释放的影响。SRIF对促甲状腺激素释放激素（TRH）诱导的TSH及其亚基的释放具有剂量依赖性抑制作用，半数有效剂量为3×10⁻⁸M。这种作用具有时间依赖性，对TSH的作用比对其亚基的作用更强。然而，SRIF对TSH及其亚基在24小时内的基础释放和总产量没有影响。在抑制TRH诱导的TSH及其亚基释放方面，SRIF的作用与甲状腺激素的作用具有相加性。SRIF与甲状腺激素联合使用可完全抑制TRH诱导的TSH及其亚基的释放。相反，甲状腺激素对TRH诱导的TSH及其亚基的总产量和释放具有剂量依赖性抑制作用。此外，甲状腺激素使单剂量SRIF抑制TRH诱导的TSH释放的效力呈剂量依赖性增加（r = 0.81；P＜0.001）。对这些数据的分析表明，甲状腺激素与SRIF的作用协同抑制TRH介导的TSH及其亚基的释放。
