来自蜡样芽孢杆菌的第三种不具有ATP依赖性DNA酶活性的DNA依赖性ATP酶。

A third DNA-dependent ATPase from Bacillus cereus free of ATP-dependent DNase activity.

作者信息

Ohlbaum A, Csuzi S, Antoni F, Banfalvi G

出版信息

FEBS Lett. 1983 Jul 11;158(1):63-7. doi: 10.1016/0014-5793(83)80677-2.

Abstract

The purification of ATP-dependent DNase from Bacillus cereus led to the isolation and characterization of a third DNA-dependent ATPase. The enzyme called ATPase III has been purified free of nuclease activity. None of the expected ATPases proved to be identical with ATP-dependent DNase-DNA-dependent ATPase. Separation of ATPase I, II and III and a DNase specific for single-stranded DNA from the same source excludes the possibility of ATP-dependent DNase being the action of a single enzyme molecule.

摘要

从蜡样芽孢杆菌中纯化ATP依赖性脱氧核糖核酸酶，导致了第三种DNA依赖性ATP酶的分离和特性鉴定。这种被称为ATP酶III的酶已被纯化至无核酸酶活性。结果表明，预期的ATP酶中没有一种与ATP依赖性脱氧核糖核酸酶（即DNA依赖性ATP酶）相同。从同一来源分离出ATP酶I、II和III以及一种对单链DNA具有特异性的脱氧核糖核酸酶，排除了ATP依赖性脱氧核糖核酸酶是单一酶分子作用的可能性。