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Resolution and reconstitution of the rec BC deoxyribonuclease of Escherichia coli.

作者信息

Banfalvi G, Csuzi S, Antoni F

出版信息

FEBS Lett. 1983 Nov 28;164(1):28-32. doi: 10.1016/0014-5793(83)80012-x.

DOI:10.1016/0014-5793(83)80012-x
PMID:6228438
Abstract

The inactivation of rec BC nuclease activity and simultaneously the separation of 3 DNA-dependent ATPases and an ATP-independent DNases specific for single-stranded DNA have been observed after DEAE-cellulose chromatography of cell extracts from Escherichia coli. Two of the ATPases catalyze the strand separation of duplex DNA. Reconstitution of ATP-dependent DNase activity has been carried out by the combination of the separated enzymes.

摘要

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