Kotulla P, Kostner C, Schleusener H
J Endocrinol Invest. 1983 Apr;6(2):135-8. doi: 10.1007/BF03350586.
A radioligand receptor assay (RRA) for TSH and TSH-binding-inhibiting antibodies (TBIAb) using solubilized receptor is presented. Introducing charcoal for separation of bound and free hormone has made the assay easier to handle. The detection limit for TSH is 30 microU/ml as measured by RIA. TBIAb was found in the sera of 19 of 26 hyperthyroid patients. Only 16 sera were positive when the RRA using membrane fragments was applied.
本文介绍了一种使用溶解受体的促甲状腺激素（TSH）和促甲状腺激素结合抑制抗体（TBIAb）的放射性配体受体分析（RRA）方法。引入活性炭用于分离结合态和游离态激素，使该分析操作更简便。用放射免疫分析（RIA）测得TSH的检测限为30微单位/毫升。在26例甲状腺功能亢进患者的血清中，有19例检测到TBIAb。当应用使用膜片段的RRA时，只有16份血清呈阳性。
