Ralph P, Nakoinz I
J Immunol. 1983 Aug;131(2):1028-31.
Seven murine monoclonal antibodies to antigens expressed on T lymphoma targets were tested for directing antibody-dependent cellular cytotoxicity (ADCC). Peptone-induced peritoneal exudate macrophages, the LPS-stimulated RAW264.10 cell line, and human blood nonadherent mononuclear leukocytes were used as effector cells. All six IgG monoclonals tested, representing the four murine IgG isotypes and directed against four antigens (Thy-1.2, H-2k, Ly-2.1, Ly-9.2), were all active in ADCC. In contrast, an IgM anti-Thy-1.2 showed no activity despite very high C-cytotoxic titers. Thus, there does not seem to be any restriction among IgG classes for directing ADCC to tumor targets mediated by murine macrophages or human K cells.
对七种针对T淋巴瘤靶标上表达的抗原的鼠单克隆抗体进行了指导抗体依赖性细胞毒性（ADCC）的测试。用蛋白胨诱导的腹腔渗出巨噬细胞、脂多糖刺激的RAW264.10细胞系和人血非贴壁单核白细胞作为效应细胞。所测试的六种IgG单克隆抗体，代表四种鼠IgG同种型并针对四种抗原（Thy-1.2、H-2k、Ly-2.1、Ly-9.2），在ADCC中均具有活性。相比之下，一种抗Thy-1.2 IgM尽管具有非常高的细胞毒性滴度，但没有活性。因此，在由鼠巨噬细胞或人K细胞介导的将ADCC导向肿瘤靶标的过程中，IgG类别之间似乎没有任何限制。
