一种针对人肝细胞癌相关抗原上碳水化合物链的单克隆抗体，该抗体可抑制裸鼠体内肿瘤生长。
A monoclonal antibody to the carbohydrate chain on human hepatocellular carcinoma-associated antigen which suppressed tumor growth in nude mice.
作者信息
Fukuda Y, Imai K, Miura K, Matsui M, Nakanishi T, Nakazato H, Masukawa J, Higashide T, Hinoda Y, Noguchi T
机构信息
Suntory Institute for Biomedical Research, Osaka, Japan.
出版信息
Cancer Immunol Immunother. 1988;27(1):26-32. doi: 10.1007/BF00205754.
There have been few reports stating that monoclonal antibody alone inhibits human solid tumor growth in vivo. The present study demonstrated that monoclonal antibody S1 (IgG2a), which recognized the antigenic determinant of the carbohydrate moiety, showed antibody-dependent cell (or macrophage)-mediated cytotoxicity (ADCC or ADMC) in conjunction with murine splenocytes of both BALB/c and athymic mice. In vivo experiments demonstrated that the antibody S1 clearly prolonged the survival of athymic mice which had been inoculated with a human liver carcinoma cell line. In addition, the antibody S1 significantly suppressed the human hepatoma line transplanted s.c. into nude mice. 125I-Labeled monoclonal antibody S1 revealed that the antibody accumulated significantly in the tumor mass. Many mononuclear cells were observed surrounding tumor cells when the antibody was given. This model system might be useful for analyzing the ADCC (or ADMC) mechanism in vivo.
很少有报道称单克隆抗体单独就能在体内抑制人类实体瘤生长。本研究表明，识别碳水化合物部分抗原决定簇的单克隆抗体S1（IgG2a），与BALB/c小鼠和无胸腺小鼠的鼠脾细胞共同作用时，表现出抗体依赖性细胞（或巨噬细胞）介导的细胞毒性（ADCC或ADMC）。体内实验表明，抗体S1明显延长了接种人肝癌细胞系的无胸腺小鼠的生存期。此外，抗体S1显著抑制了皮下移植到裸鼠体内的人肝癌细胞系。125I标记的单克隆抗体S1显示该抗体在肿瘤块中显著蓄积。给予抗体时，观察到肿瘤细胞周围有许多单核细胞。该模型系统可能有助于分析体内的ADCC（或ADMC）机制。
Zotero 插件