Feilberg Jørgensen N H, Møller J K, Stenderup A
J Gen Microbiol. 1982 Dec;128(12):2991-6. doi: 10.1099/00221287-128-12-2991.
The indirect haemagglutination reaction was evaluated in the classification of conjugative plasmids. A simple and sensitive method was worked out using pili and in some cases whole bacteria as antigens. Antibodies were prepared against pili coded for by plasmids from incompatibility groups IncFI, IncFII, IncI alpha and IncN. The antisera were tested against pili from 35 strains harbouring plasmids. The test differentiated clearly between plasmids from unrelated incompatibility groups, whereas cross-reaction occurred with closely related groups such as FI and FII. Minor antigenic variation could be seen within the IncFII group.
在接合质粒的分类中对间接血凝反应进行了评估。利用菌毛并在某些情况下使用全菌作为抗原，制定了一种简单且灵敏的方法。制备了针对来自不相容群IncFI、IncFII、IncIα和IncN的质粒所编码菌毛的抗体。用这些抗血清检测了来自35株携带质粒菌株的菌毛。该试验能清晰地区分来自不相关不相容群的质粒，而对于密切相关的群如FI和FII则会发生交叉反应。在IncFII群内可见轻微的抗原变异。
