The high affinity uptake of GABA in optic tectum was found to be about 40% higher in frogs kept in complete darkness for 3 weeks, than in frogs in the normal condition. 2. No effects were obtained in uptake of glutamate and activity of GAD in the optic tectum. 3. The isoenzyme composition of cholinesterases in frog optic tectum and retina was not affected by dark adaptation either.