Bories G F, Peleran J C, Wal J M, Corpet D E
Drug Metab Dispos. 1983 May-Jun;11(3):249-54.
Simple and ion-pair reverse phase high performance chromatographic separations combined with selective extraction were developed in order to achieve a qualitative and quantitative analysis of [3H]chloramphenicol (CP) metabolites in rat urine. Complete separation followed by unequivocal identification of all the metabolites was obtained, especially of the toxicologically significant CP-arylamine. Metabolic profiles were established from human and goat urine which confirmed data already available, but was used to identify new acidic metabolites in the latter species. This methodology appears to be a useful research tool in comparative metabolic studies.
为了对大鼠尿液中[3H]氯霉素（CP）代谢物进行定性和定量分析，开发了简单的离子对反相高效色谱分离法并结合选择性萃取。实现了完全分离，随后明确鉴定了所有代谢物，尤其是具有毒理学意义的CP-芳胺。从人尿和山羊尿中建立了代谢谱，这证实了已有的数据，但用于鉴定后一种物种中的新酸性代谢物。这种方法似乎是比较代谢研究中一种有用的研究工具。
