α2-、β1-和β2-肾上腺素能受体对大鼠下丘脑切片内源性去甲肾上腺素和多巴胺释放的突触前介导作用。
Presynaptic mediation by alpha 2-, beta 1- and beta 2-adrenoceptors of endogenous noradrenaline and dopamine release from slices of rat hypothalamus.
作者信息
Ueda H, Goshima Y, Misu Y
出版信息
Life Sci. 1983 Jul 25;33(4):371-6. doi: 10.1016/s0024-3205(83)80011-3.
Using high performance liquid chromatography with an electro-chemical detector, we studied effects of different compounds on the impulse-evoked release of endogenous noradrenaline (NA) and dopamine (DA) release from slices of the rat hypothalamus. Adrenaline (10(-7) M), with a potent alpha-agonistic action decreased both NA and DA release, and these effects were blocked by pretreatment with yohimbine (10(-7 M). The alpha 2-antagonist, yohimbine alone (10(-8) - 10(-6) M) concentration-dependently increased these releases, while alpha 1-antagonist, prazosin showed weak increase on NA but not DA release at 10(-6) M. Isoproterenol (10(-10) - 10(-8) M) concentration-dependently increased these releases and the effects were antagonized by pretreatment with a non-selective beta-antagonist, 1-propranolol, a beta 1-antagonist, atenolol or a beta 2-antagonist, butoxamine. 1-Propranolol (3 X 10(-7) M) alone, but not the d-isomer inhibited the releases. Thus, in the rat hypothalamus, the release of NA and DA may be mediated via presynaptic alpha 2-, beta 1- and beta 2-adrenoceptors.
我们使用配备电化学检测器的高效液相色谱法，研究了不同化合物对大鼠下丘脑切片中内源性去甲肾上腺素（NA）和多巴胺（DA）冲动诱发释放的影响。具有强效α激动作用的肾上腺素（10⁻⁷ M）降低了NA和DA的释放，而育亨宾（10⁻⁷ M）预处理可阻断这些作用。α₂拮抗剂育亨宾单独使用（10⁻⁸ - 10⁻⁶ M）时，可浓度依赖性地增加这些释放，而α₁拮抗剂哌唑嗪在10⁻⁶ M时对NA释放有微弱增加，但对DA释放无影响。异丙肾上腺素（10⁻¹⁰ - 10⁻⁸ M）浓度依赖性地增加这些释放，且这些作用可被非选择性β拮抗剂普萘洛尔、β₁拮抗剂阿替洛尔或β₂拮抗剂布托沙明预处理所拮抗。单独使用普萘洛尔（3×10⁻⁷ M），而非其d-异构体，可抑制释放。因此，在大鼠下丘脑中，NA和DA的释放可能通过突触前α₂、β₁和β₂肾上腺素能受体介导。
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