Dupé-Godet M, Adjovi Y
Comp Biochem Physiol A Comp Physiol. 1983;75(3):347-52. doi: 10.1016/0300-9629(83)90092-0.
In the Varanus exanthematicus, the pancreatic complex comprises the true pancreas as well as an intrasplenic islet comparable to a Brockmann's body. Somatostatin content and concentration were estimated by radioimmunoassay in acetic acid extracts of both organs. Relatively large amounts of somatostatin (SLI) are present in the pancreas (2.17 +/- 0.07 micrograms) without any difference in distribution between the cranial (CP) and mediocaudal (MCP) regions. The intrasplenic islet contains as much SLI material as the whole pancreas (3.38 +/- 0.85 microgram); thus, this primitive organ presents a very high hormonal concentration (109.34 +/- 40.30 ng/mg wt). Serial dilutions of the extracts gave parallel immunoassay displacement curves and gel-filtration revealed two immunoreactive peaks: the most important one was found in the synthetic tetradecapeptide fraction, the other one near the void volume fraction. These results show an immunological similarity between the SLI substance of the pancreatic complex and the synthetic somatostatin; however, a molecular heterogeneity must not be excluded. The results are discussed from a phylogenetic point of view.
在砂巨蜥中，胰腺复合体包括真正的胰腺以及一个与布罗克曼体类似的脾内胰岛。通过放射免疫分析法对这两个器官的醋酸提取物中的生长抑素含量和浓度进行了测定。胰腺中存在相对大量的生长抑素（SLI）（2.17±0.07微克），头侧（CP）和中尾侧（MCP）区域之间的分布没有差异。脾内胰岛所含的SLI物质与整个胰腺一样多（3.38±0.85微克）；因此，这个原始器官呈现出非常高的激素浓度（109.34±40.30纳克/毫克湿重）。提取物的系列稀释给出了平行的免疫分析置换曲线，凝胶过滤显示出两个免疫反应峰：最重要的一个出现在合成十四肽部分，另一个在空体积部分附近。这些结果表明胰腺复合体的SLI物质与合成生长抑素之间存在免疫学相似性；然而，不能排除分子异质性。从系统发育的角度对结果进行了讨论。
