Hattingh J, Bomzon L, Marcus E, Jooste C, Ganhao M F, Cheney C S, de Vos V
Comp Biochem Physiol A Comp Physiol. 1983;75(3):441-5. doi: 10.1016/0300-9629(83)90107-x.
Total protein concentrations from a variety of wild mammalian species were measured by the Lowry and Biuret techniques and compared with results obtained or derived by refractometry. Values obtained with the Lowry method were the highest and certain derived refractometry results, the lowest. It is suggested that the Biuret method provides the most acceptable values but that care should be exercised in using the correct standard. The percentage composition of plasma from 22 species of wild mammals is also presented and the observations discussed with relation to methodology and A/G ratio.
采用洛瑞法和双缩脲法测定了多种野生哺乳动物的总蛋白浓度,并与折射法获得或推导的结果进行了比较。用洛瑞法得到的值最高,而某些推导的折射法结果最低。建议双缩脲法提供的数值最可接受,但在使用正确标准时应谨慎。还给出了22种野生哺乳动物血浆的百分比组成,并就方法学和A/G比值对观察结果进行了讨论。