Jégou B, Laws A O, de Kretser D M
J Reprod Fertil. 1983 Sep;69(1):137-45. doi: 10.1530/jrf.0.0690137.
Cryptorchidism for 28 or 10 days resulted in a severe disruption of spermatogenesis (assessed histologically or by fertility tests), Sertoli cell function (assessed by seminiferous tubule fluid production after efferent duct ligation, ABP levels, binding of 125I-labelled FSH to testis homogenates and serum FSH levels) and Leydig cell function (assessed by serum LH and testosterone levels, in-vitro testosterone production, binding of 125I-labelled hCG). Orchidopexy after 28 days of cryptorchidism resulted in a poor recovery of spermatogenesis since the majority of tubules were lined by Sertoli cells and a few spermatogonia. No recovery occurred in the indicators of Sertoli and Leydig cell function. Orchidopexy after 10 days of cryptorchidism also resulted in a poor recovery of spermatogenesis, with a few animals showing partial recovery after 6 months. No recovery occurred in seminiferous tubule fluid production but partial recovery occurred in ABP content and production rate. Serum FSH, LH levels and in-vitro testosterone production by the testis remained elevated and did not change from the values found during cryptorchidism. Fertility testing at 6 months revealed a small number of rats in which fertility was restored although the number of embryos was lower than in controls. In this group of animals there was a significant improvement in a number of indicators of Sertoli cell and Leydig cell function. These data provide further evidence to link the changes in Sertoli cell and Leydig cell function to the germ cell complement present in the testis.
隐睾28天或10天会导致精子发生严重受损（通过组织学评估或生育力测试）、支持细胞功能受损（通过结扎输出小管后曲细精管液生成、ABP水平、125I标记的FSH与睾丸匀浆的结合以及血清FSH水平来评估）和间质细胞功能受损（通过血清LH和睾酮水平、体外睾酮生成、125I标记的hCG结合来评估）。隐睾28天后进行睾丸固定术，精子发生恢复不佳，因为大多数曲细精管由支持细胞和少数精原细胞构成。支持细胞和间质细胞功能指标未恢复。隐睾10天后进行睾丸固定术，精子发生恢复也不佳，少数动物在6个月后有部分恢复。曲细精管液生成未恢复，但ABP含量和生成率有部分恢复。血清FSH、LH水平以及睾丸体外睾酮生成仍升高，且与隐睾期间的值无变化。6个月时的生育力测试显示，虽有少数大鼠恢复了生育力，但胚胎数量低于对照组。在这组动物中，支持细胞和间质细胞功能的一些指标有显著改善。这些数据进一步证明了支持细胞和间质细胞功能的变化与睾丸中存在的生殖细胞数量有关。
