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非洲爪蟾卵母细胞中外源信使核糖核酸翻译的单个γ-氨基丁酸和乙酰胆碱激活受体通道的记录。

Recording of single gamma-aminobutyrate- and acetylcholine-activated receptor channels translated by exogenous mRNA in Xenopus oocytes.

作者信息

Miledi R, Parker I, Sumikawa K

出版信息

Proc R Soc Lond B Biol Sci. 1983 Jul 22;218(1213):481-4. doi: 10.1098/rspb.1983.0053.

DOI:10.1098/rspb.1983.0053
PMID:6136979
Abstract

High resolution ('giga-seal') patch clamp recording in Xenopus oocytes was used to measure single channel currents from ACh- and GABA-activated receptors. The proteins that make up these receptors had been translated from mRNA derived from, respectively, denervated cat muscle and chick optic lobe.

摘要

利用非洲爪蟾卵母细胞中的高分辨率（“千兆封接”）膜片钳记录技术来测量乙酰胆碱和γ-氨基丁酸激活受体的单通道电流。构成这些受体的蛋白质分别是由取自去神经支配的猫肌肉和鸡视叶的信使核糖核酸翻译而来的。

相似文献

1
Recording of single gamma-aminobutyrate- and acetylcholine-activated receptor channels translated by exogenous mRNA in Xenopus oocytes.非洲爪蟾卵母细胞中外源信使核糖核酸翻译的单个γ-氨基丁酸和乙酰胆碱激活受体通道的记录。
Proc R Soc Lond B Biol Sci. 1983 Jul 22;218(1213):481-4. doi: 10.1098/rspb.1983.0053.
2
Properties of acetylcholine receptors translated by cat muscle mRNA in Xenopus oocytes.非洲爪蟾卵母细胞中由猫肌肉信使核糖核酸翻译的乙酰胆碱受体的特性
EMBO J. 1982;1(11):1307-12. doi: 10.1002/j.1460-2075.1982.tb01315.x.
3
Acetylcholine and GABA receptors: subunits of central and peripheral receptors and their encoding nucleic acids.乙酰胆碱和γ-氨基丁酸受体：中枢和外周受体的亚基及其编码核酸。
Cold Spring Harb Symp Quant Biol. 1983;48 Pt 1:109-24. doi: 10.1101/sqb.1983.048.01.014.
4
Expression of ACh-activated channels and sodium channels by messenger RNAs from innervated and denervated muscle.来自受神经支配和去神经支配肌肉的信使核糖核酸对乙酰胆碱激活通道和钠通道的表达。
Proc R Soc Lond B Biol Sci. 1988 Apr 22;233(1272):235-46. doi: 10.1098/rspb.1988.0021.
5
Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes.编码烟碱型乙酰胆碱受体的外源信使核糖核酸在非洲爪蟾卵母细胞中翻译产生功能性受体。
Proc R Soc Lond B Biol Sci. 1982 May 22;215(1199):241-6. doi: 10.1098/rspb.1982.0040.
6
Change in desensitization of cat muscle acetylcholine receptor caused by coexpression of Torpedo acetylcholine receptor subunits in Xenopus oocytes.非洲爪蟾卵母细胞中枪乌贼乙酰胆碱受体亚基共表达导致的猫肌肉乙酰胆碱受体脱敏变化。
Proc Natl Acad Sci U S A. 1989 Jan;86(1):367-71. doi: 10.1073/pnas.86.1.367.
7
Synthesis of chick brain GABA receptors by frog oocytes.蛙卵对鸡脑γ-氨基丁酸受体的合成
Proc R Soc Lond B Biol Sci. 1982 Nov 22;216(1205):509-15. doi: 10.1098/rspb.1982.0089.
8
Glutamate and kainate receptors induced by rat brain messenger RNA in Xenopus oocytes.大鼠脑信使核糖核酸在非洲爪蟾卵母细胞中诱导产生的谷氨酸和红藻氨酸受体。
Proc R Soc Lond B Biol Sci. 1984 Apr 24;221(1223):127-43. doi: 10.1098/rspb.1984.0027.
9
Expression of functional GABA, glycine and glutamate receptors in Xenopus oocytes injected with rat brain mRNA.在注射大鼠脑信使核糖核酸的非洲爪蟾卵母细胞中功能性γ-氨基丁酸、甘氨酸和谷氨酸受体的表达
Nature. 1984;310(5975):318-21. doi: 10.1038/310318a0.
10
Blockage of nicotinic acetylcholine receptors by 5-hydroxytryptamine.5-羟色胺对烟碱型乙酰胆碱受体的阻断作用。
J Neurosci Res. 1993 Apr 1;34(5):562-70. doi: 10.1002/jnr.490340508.

引用本文的文献

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A half century of γ-aminobutyric acid.γ-氨基丁酸的半个世纪
Brain Neurosci Adv. 2019 Nov 27;3:2398212819858249. doi: 10.1177/2398212819858249. eCollection 2019 Jan-Dec.
2
Oocyte's Conductance for Bioactive Compounds Screening and Characterization.卵母细胞对生物活性化合物的传导筛选与特性分析
Int J Mol Sci. 2019 Apr 27;20(9):2083. doi: 10.3390/ijms20092083.
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Evaluating the longevity of surgically extracted Xenopus laevis oocytes for the study of nematode ligand-gated ion channels.评估用于线虫配体门控离子通道研究的手术提取非洲爪蟾卵母细胞的寿命。
Invert Neurosci. 2017 Nov 28;18(1):1. doi: 10.1007/s10158-017-0205-z.
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The Xenopus oocyte: a single-cell model for studying Ca2+ signaling.非洲爪蟾卵母细胞：用于研究钙离子信号传导的单细胞模型。
Cold Spring Harb Protoc. 2013 Mar 1;2013(3):10.1101/pdb.top066308 pdb.top066308. doi: 10.1101/pdb.top066308.
5
Strychnine activates neuronal alpha7 nicotinic receptors after mutations in the leucine ring and transmitter binding site domains.士的宁在亮氨酸环和递质结合位点结构域发生突变后激活神经元α7烟碱型受体。
Proc Natl Acad Sci U S A. 1999 Nov 9;96(23):13421-6. doi: 10.1073/pnas.96.23.13421.
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Two-microelectrode voltage clamp of Xenopus oocytes: voltage errors and compensation for local current flow.非洲爪蟾卵母细胞的双微电极电压钳制：电压误差与局部电流流动的补偿
Biophys J. 1999 Oct;77(4):1980-91. doi: 10.1016/S0006-3495(99)77039-6.
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Effects of Zn2+ on wild and mutant neuronal alpha7 nicotinic receptors.锌离子对野生型和突变型神经元α7烟碱型受体的影响。
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10246-50. doi: 10.1073/pnas.95.17.10246.
8
Neuronal nicotinic threonine-for-leucine 247 alpha7 mutant receptors show different gating kinetics when activated by acetylcholine or by the noncompetitive agonist 5-hydroxytryptamine.神经元烟碱型苏氨酸取代亮氨酸247的α7突变受体在被乙酰胆碱或非竞争性激动剂5-羟色胺激活时表现出不同的门控动力学。
Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9915-9. doi: 10.1073/pnas.94.18.9915.
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Blockage of muscle and neuronal nicotinic acetylcholine receptors by fluoxetine (Prozac).氟西汀（百忧解）对肌肉和神经元烟碱型乙酰胆碱受体的阻断作用。
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Partial purification and functional expression of brain mRNAs coding for neurotransmitter receptors and voltage-operated channels.编码神经递质受体和电压门控通道的脑信使核糖核酸的部分纯化及功能表达
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