二价阳离子对核蛋白提取的影响。

Effect of divalent cations on the extraction of nuclear proteins.

作者信息

Kellermayer M, Ludány A, Jobst K, Busch H

出版信息

Acta Biochim Biophys Acad Sci Hung. 1977;12(4):353-9.

Abstract

TSCM-(0.01 M Tris-HCl, 0.25 sucrose, 3.8 mM CaCl2, 12 mM MgCl2, pH 7.2) and TS-(0.01 M Tris-HCl, 0.25 M sucrose, pH 7.2)-soluble proteins of isolated rat liver nuclei were analyzed by a two-dimensional polyacrylamide gel electrophoresis method. Light microscopy showed that treatment with the TSCM solution did not alter the morphology of isolated nuclei. After treatment with the divalent cation-free TS solution, the isolated nuclei were swollen; some were disrupted and clumped together. The TSCM solution extracted 20 additional proteins including histones. These results suggest that Ca++ and Mg++ play an intracellular role in the interactions between nuclear proteins and chromatin.

摘要

采用二维聚丙烯酰胺凝胶电泳法分析了用TSCM（0.01 M Tris-HCl、0.25蔗糖、3.8 mM氯化钙、12 mM氯化镁，pH 7.2）和TS（0.01 M Tris-HCl、0.25 M蔗糖，pH 7.2）处理的分离大鼠肝细胞核的可溶性蛋白质。光学显微镜显示，用TSCM溶液处理不会改变分离细胞核的形态。用不含二价阳离子的TS溶液处理后，分离的细胞核肿胀；一些细胞核破裂并聚集在一起。TSCM溶液额外提取了20种蛋白质，包括组蛋白。这些结果表明，Ca++和Mg++在核蛋白与染色质之间的相互作用中发挥细胞内作用。