Kellermayer M, Timár T, Jobst K, Komáromy L, Trombitás K
Eur J Cell Biol. 1980 Dec;23(1):204-7.
Rat liver nuclei were isolated in the presence of a nonionic detergent, and the soluble proteins were extracted from them using low-salt solution. The isolated nuclei were then embedded in a fibrin clot which was cut into 4 to 5 mm3 pieces and placed into an AMICON pressure cell in the presence and absence of the soluble nuclear proteins. When the soluble nuclear proteins were present and when their concentrations reached 40 mg/ml, the nuclei were significantly larger and their chromatin more decondensed than in the absence of added proteins. Our findings suggest that the soluble nuclear proteins play a role in the regulation of nuclear volume and the hydration of chromatin.
在非离子去污剂存在的情况下分离大鼠肝细胞核，并用低盐溶液从其中提取可溶性蛋白质。然后将分离出的细胞核包埋在纤维蛋白凝块中，将凝块切成4至5立方毫米的小块，并在有和没有可溶性核蛋白的情况下放入AMICON压力室中。当存在可溶性核蛋白且其浓度达到40毫克/毫升时，细胞核明显比未添加蛋白质时更大，其染色质的凝聚程度更低。我们的研究结果表明，可溶性核蛋白在细胞核体积调节和染色质水合作用中发挥作用。
