Antigens in whooping cough vaccine and antibody levels induced by vaccination of children.

The content of 3 antigens--filamentous haemagglutinin, lymphocytosis-promoting factor, and serotype-specific agglutinogens (fimbriae)--was determined in the current UK whole-cell whooping cough vaccine. Antibodies to these antigens and to outer membrane proteins and lipopolysaccharide of Bordetella pertussis were measured in the serum of unvaccinated children and children who had received 1, 2, or 3 doses of the vaccine. Children who had received one dose of vaccine had varied low antibody titres. Children who had received two or three doses had significantly higher antibody titres to all the antigens tested, as did some unvaccinated children with no history of whooping cough. This study shows that filamentous haemagglutinin, lymphocytosis promoting factor, and outer membrane proteins are immunogenic constituents of the whole-cell vaccine. Their inclusion in a subcellular vaccine would not involve novel antigens.