福莫特罗放射免疫分析方法的研发。

The development of a radioimmunoassay for formoterol.

作者信息

Yokoi K, Murase K, Shiobara Y

出版信息

Life Sci. 1983 Oct 24;33(17):1665-72. doi: 10.1016/0024-3205(83)90722-1.

Abstract

The development of a radioimmunoassay (RIA) for the beta 2-stimulant formoterol is described. The sensitivity of the method is 0.1 ng/ml in plasma and urine, when a 1-ml sample is used. The cross-reactivity of the antiserum with formoterol glucuronide was 30%. Since formoterol is metabolized extensively to formoterol glucuronide in rats, dogs and humans, extraction with ethyl ether prior to the radioimmunoassay was carried out. Satisfactory agreement was obtained for levels of formoterol in plasma and urine when they were determined by RIA and gas chromatography-mass spectrometry. The concentration of formoterol was determined in dog plasma and human urine after oral administration of formoterol fumarate to dogs (61 mcg/kg) and humans (40 mcg).

摘要

本文描述了一种用于β2-兴奋剂福莫特罗的放射免疫分析（RIA）方法。当使用1毫升样本时，该方法在血浆和尿液中的灵敏度为0.1纳克/毫升。抗血清与福莫特罗葡萄糖醛酸苷的交叉反应率为30%。由于福莫特罗在大鼠、狗和人类体内会广泛代谢为福莫特罗葡萄糖醛酸苷，因此在放射免疫分析之前用乙醚进行提取。当通过放射免疫分析和气相色谱-质谱法测定血浆和尿液中福莫特罗的水平时，结果获得了令人满意的一致性。在给狗（61微克/千克）和人类（40微克）口服富马酸福莫特罗后，测定了狗血浆和人类尿液中福莫特罗的浓度。