van den Berg B T, Portier E J, van den Berg M, Braat M C, van Boxtel C J
Department of Clinical Pharmacology, Academic Medical Centre, Amsterdam, The Netherlands.
Ther Drug Monit. 1994 Apr;16(2):196-9. doi: 10.1097/00007691-199404000-00015.
A method for the assay of plasma concentrations of the long-acting beta 2-adrenoceptor agonist, formoterol is described. This method, in which high-performance liquid chromotography is used with electrochemical detection, enables, for the first time, pharmacokinetic and pharmacodynamic research with this drug. After column extraction of plasma samples, the eluent is injected on the chromatographic system. Retention times of formoterol and the internal standard, bromo-formoterol, were 5.6 and 10 min, respectively. Our results show that this is a sensitive and reproducible method with a very low limit of detection (20 pg/ml), which creates the possibility of measuring concentrations in a range achievable in humans.
描述了一种测定长效β2-肾上腺素能受体激动剂福莫特罗血浆浓度的方法。该方法采用高效液相色谱结合电化学检测，首次实现了对该药物的药代动力学和药效学研究。血浆样品经柱萃取后，将洗脱液注入色谱系统。福莫特罗和内标溴福莫特罗的保留时间分别为5.6分钟和10分钟。我们的结果表明，这是一种灵敏且可重复的方法，检测限极低（20 pg/ml），能够测定人体可达到浓度范围内的浓度。
