Snead M L, Zeichner-David M, Chandra T, Robson K J, Woo S L, Slavkin H C
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7254-8. doi: 10.1073/pnas.80.23.7254.
The determination of the biochemical phenotype of tooth epithelium requires specification by the dental mesenchyme. This is a general feature of epithelial-mesenchymal interaction in a number of different epidermal organ systems (e.g., salivary gland, mammary gland, feather, skin, and hair morphogenesis). To investigate these developmental processes, we have identified a cDNA clone representing the major group of gene products associated with enamel extracellular matrix formation. The mRNAs for mouse amelogenins, representing approximately equal to 90% of the total enamel proteins, have been isolated and partially characterized by specific immunoprecipitation. The poly(A)-containing RNAs were used for the synthesis and cloning of the mouse amelogenin cDNA. Recombinant plasmids containing amelogenin cDNA sequences were identified by differential hybridization, hybrid-selected translation, and blot hybridization analyses. A cloned sequence was used to identify the expression of amelogenins during tooth development. The mouse cDNA sequence hybridized to genomic mouse and human DNAs. This amelogenin cDNA probe now enables molecular investigations of a number of classical problems in developmental biology.
牙齿上皮细胞生化表型的确定需要牙间充质的特异性作用。这是许多不同表皮器官系统（如唾液腺、乳腺、羽毛、皮肤和毛发形态发生）中上皮-间充质相互作用的一个普遍特征。为了研究这些发育过程，我们鉴定了一个cDNA克隆，它代表了与釉质细胞外基质形成相关的主要基因产物组。已分离出代表约90%总釉质蛋白的小鼠釉原蛋白mRNA，并通过特异性免疫沉淀对其进行了部分表征。含poly(A)的RNA用于小鼠釉原蛋白cDNA的合成和克隆。通过差异杂交、杂交选择翻译和印迹杂交分析鉴定了含有釉原蛋白cDNA序列的重组质粒。一个克隆序列用于鉴定牙齿发育过程中釉原蛋白的表达。小鼠cDNA序列与基因组小鼠和人类DNA杂交。这种釉原蛋白cDNA探针现在能够对发育生物学中的一些经典问题进行分子研究。
