Enzyme-linked immunosorbent assay (ELISA) and colorimetric determination of cow gamma-glutamyltransferase.

Using specific antibodies of anti-heavy and light forms of bovine kidney gamma-glutamyltransferase, both enzyme forms were determined in some cow body fluids and tissue preparations by enzyme linked immunosorbent assay (ELISA). The mean activity of the light form of gamma-glutamyltransferase in cow sera, new-born calf sera and in cow colostra was 29.2, 612 and 2630 mU/ml respectively. Good correlation was noted between the results obtained by ELISA and by colorimetric method. In supernatants from cow kidney and liver homogenate after incubation at 37 degrees C, a marked increase of the heavy form assayed by ELISA was noted.