Bossmann B, Haschen R J
J Clin Chem Clin Biochem. 1983 Nov;21(11):659-63. doi: 10.1515/cclm.1983.21.11.659.
Results obtained with the in vitro Wilson-Wiseman method [1954), J. Physiol. 123, 116-125) and with an in vivo perfusion model are compared. The in vitro method suffers from insufficient oxygenation. Five plasma membrane enzymes, five cytosol and two lysosomal enzymes were estimated. The medium consisted of Ringer solution without and with 10 mmol/l taurocholate. The greatest difference between both models is shown by the lysosomal enzymes, which are amply released in vitro but not in vivo. The in vitro to in vivo ratios of the release rates of cytosol enzymes vary from about 10 to less than one suggesting deterioration by lysosomal cathepsins in certain cases. The removal of plasma membrane enzymes, particularly those that are characterized by superficial localization, is inhibited by hypoxia. This implies that the normal release of these enzymes, at least in part, is energy-dependent.
将采用体外威尔逊-怀斯曼方法[1954年，《生理学杂志》123卷，116 - 125页]所获得的结果与采用体内灌注模型所获得的结果进行了比较。体外方法存在氧合不足的问题。对五种质膜酶、五种胞质溶胶酶和两种溶酶体酶进行了评估。培养基由不含和含有10 mmol/L牛磺胆酸盐的林格溶液组成。两种模型之间最大的差异体现在溶酶体酶上，溶酶体酶在体外大量释放，但在体内却不会。胞质溶胶酶释放率的体外与体内比值在约10到小于1之间变化，这表明在某些情况下会被溶酶体组织蛋白酶降解。缺氧会抑制质膜酶的清除，尤其是那些具有表面定位特征的酶。这意味着这些酶的正常释放至少部分是能量依赖的。
