Shi Z R, McIntyre L J, Knowles B B, Solter D, Kim Y S
Cancer Res. 1984 Mar;44(3):1142-7.
The expression of the carbohydrate structure defined by monoclonal antibody to murine stage-specific embryonic antigen 1 (SSEA-1) was examined, using immunofluorescence, in formalin-fixed, paraffin-embedded sections of normal fetal and adult human colon and human colonic adenocarcinoma. SSEA-1 was expressed in all human colonic adenocarcinoma tissues examined, although in some cases the staining was heterogeneous. In normal human colonic mucosa, under the conditions used, faint staining was seen in the lower crypts and in only 26% of the crypts examined. When human fetal colon was tested, SSEA-1 was expressed in much larger amounts and in over 50% of all crypts. Transitional mucosa, immediately adjacent to human colonic adenocarcinomas, was also tested, and in this case, increased SSEA-1 expression was seen not only in the lower crypts but also in the upper crypts and surface epithelium. These results show that the increased expression of SSEA-1 in human colonic adenocarcinoma is an oncodevelopmental marker for this cancer. In addition, the results suggest that increased expression of SSEA-1 may be a preneoplastic change in human colon.
利用免疫荧光技术，在正常胎儿及成人结肠和人结肠腺癌的福尔马林固定、石蜡包埋切片中，检测了由抗小鼠阶段特异性胚胎抗原1（SSEA-1）单克隆抗体所定义的碳水化合物结构的表达。在所检测的所有人类结肠腺癌组织中均有SSEA-1表达，不过在某些情况下染色是不均匀的。在正常人类结肠黏膜中，在所采用的条件下，在隐窝下部可见微弱染色，且在所检测的隐窝中仅有26%出现这种情况。对人类胎儿结肠进行检测时，SSEA-1表达量更多，且在所有隐窝的50%以上有表达。紧邻人结肠腺癌的移行黏膜也进行了检测，在这种情况下，不仅在隐窝下部，而且在隐窝上部和表面上皮中均可见SSEA-1表达增加。这些结果表明，SSEA-1在人结肠腺癌中的表达增加是该癌症的一种肿瘤发生发展标志物。此外，这些结果提示，SSEA-1表达增加可能是人类结肠的一种肿瘤前变化。
