Wolf B C, Salem R R, Sears H F, Horst D A, Lavin P T, Herlyn M, Itzkowitz S H, Schlom J, Steele G D
Department of Surgery, New England Deaconess Hospital, Boston, MA 02115.
Am J Pathol. 1989 Jul;135(1):111-9.
Immunohistologic studies were performed to evaluate the expression and regional distribution of 20 colorectal carcinoma-associated antigens in the colonic mucosa of 12 normal adults. A distinct regional variation was seen in the expression of blood group A, B, Lewis(b), and extended Lewis(y) antigens, which were expressed predominantly in the right colon, whereas Lewis(a), Lewis(y), and H-type 2 were prevalent throughout. Lewis(x) and X-like antigens were only occasionally expressed. Two antibodies against sialylated Lewis(a) showed different staining patterns, with CA19.9 positive in only two biopsies and CO29.11 intensely positive in most. Two of the three antibodies detecting carcinoembryonic antigen epitopes (3d6 and COL-4) stained many biopsies, whereas COL-12 was rarely detected. No regional gradient was found in the expression of the other antigens studied (B72.3, ME491; GA73.3, ND1, and ND4). This mapping data will provide an important baseline for future studies of epitope distribution in the colon in premalignant and neoplastic conditions.
进行免疫组织学研究以评估20种结肠直肠癌相关抗原在12名正常成年人结肠黏膜中的表达及区域分布。血型A、B、Lewis(b)和扩展Lewis(y)抗原的表达存在明显的区域差异，这些抗原主要在右半结肠表达，而Lewis(a)、Lewis(y)和H-2型在整个结肠中普遍存在。Lewis(x)和X样抗原仅偶尔表达。两种抗唾液酸化Lewis(a)的抗体显示出不同的染色模式，CA19.9仅在两份活检标本中呈阳性，而CO29.11在大多数标本中呈强阳性。检测癌胚抗原表位的三种抗体中的两种（3d6和COL-4）在许多活检标本中染色，而COL-12很少被检测到。在所研究的其他抗原（B72.3、ME491；GA73.3、ND1和ND4）的表达中未发现区域梯度。该定位数据将为未来研究癌前和肿瘤状态下结肠中表位分布提供重要的基线。
