Maro B, Paintrand M, Sauron M E, Paulin D, Bornens M
Exp Cell Res. 1984 Feb;150(2):452-8. doi: 10.1016/0014-4827(84)90589-5.
HeLa cells were examined by immunofluorescence using anti-vimentin and anti-centrosphere anti-bodies, and by transmission electron microscopy (TEM), after vimentin redistribution induced by the action of nocodazole or taxol. A redistribution of vimentin bundles in the centriolar area was observed after nocodazole treatment, although no direct interaction between centrioles and vimentin filaments could be detected. After taxol treatment, the juxtanuclear accumulation of vimentin filaments and the centrioles were rarely observed in the same area. Our results do not support the concept of a direct association between centrioles and vimentin filaments.
在用诺考达唑或紫杉醇作用诱导波形蛋白重新分布后，使用抗波形蛋白和抗中心球抗体通过免疫荧光以及通过透射电子显微镜（TEM）对HeLa细胞进行了检查。诺考达唑处理后，在中心粒区域观察到波形蛋白束的重新分布，尽管未检测到中心粒与波形蛋白丝之间的直接相互作用。紫杉醇处理后，很少在同一区域观察到波形蛋白丝和中心粒在核周的积聚。我们的结果不支持中心粒与波形蛋白丝之间存在直接关联的概念。
