处于中期 II 阻滞状态的小鼠卵母细胞中的非纺锤体微管组织中心
Non-spindle microtubule organizing centers in metaphase II-arrested mouse oocytes.
作者信息
Maro B, Howlett S K, Webb M
出版信息
J Cell Biol. 1985 Nov;101(5 Pt 1):1665-72. doi: 10.1083/jcb.101.5.1665.
Abstract
A human autoantiserum (5051) directed against pericentriolar material (PCM) was used to study the distribution of microtubule-organizing centers (MTOCs) in the oocyte and during the first cell cycle of mouse development. In oocytes, the PCM was found not only at the poles of the barrel-shaped metaphase II spindle but also at many discrete loci around the cytoplasm near the cell cortex. The spindle poles were also composed of several PCM foci. In metaphase-arrested eggs only the PCM foci located near the chromosomes acted as MTOCs. However, after reduction of the critical concentration for tubulin polymerization by taxol, the cytoplasmic PCM foci were also found to be associated with nucleation of microtubules. After fertilization the cortical PCM foci remained in a peripheral position until the end of the S phase, when they appeared to migrate centrally towards the pronuclei. At prometaphase of the first mitotic division, numerous MTOCs were found around the two sets of chromosomes; these MTOCs then aligned to form two bands on either side of the metaphase plate of the first mitosis.
摘要
一种针对中心粒周围物质（PCM）的人自身抗血清（5051）被用于研究小鼠发育过程中卵母细胞和第一个细胞周期内微管组织中心（MTOC）的分布。在卵母细胞中，不仅在桶状中期II纺锤体的两极发现了PCM，而且在靠近细胞皮质的细胞质周围的许多离散位点也发现了PCM。纺锤体两极也由几个PCM焦点组成。在中期停滞的卵中，只有位于染色体附近的PCM焦点作为MTOC起作用。然而，在用紫杉醇降低微管蛋白聚合的临界浓度后，也发现细胞质PCM焦点与微管的成核有关。受精后，皮质PCM焦点一直处于外周位置，直到S期结束，此时它们似乎向中央迁移至原核。在第一次有丝分裂的前中期，在两组染色体周围发现了许多MTOC；然后这些MTOC排列在第一次有丝分裂中期板两侧形成两条带。
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