Varma T R, Patel R H, Everard D M
Int J Fertil. 1983;28(4):243-6.
Radioimmunoassays (RIA) of serum gonadotrophins, prolactin, oestradiol, and progesterone were carried out from day 10 to 20 in 25 normal women for two cycles. In addition, we measured LH in urine twice daily using semiquantitative Hi-Gonavis kits. Finally, vaginal cytology was assessed daily in order to find out the optimum time for conception. LH in serum, as well as in urine, increased significantly at midcycle and so did FSH, prolactin, oestradiol, and the cornification index. Hi-Gonavis showed a significant increase 12 hours prior to the day of ovulation (day 0). The levels reached the peak on the day of ovulation and remained high 12 hours after the peak level on day 0. For the evaluation of the optimum time for conception in normal women who receive artificial insemination, or to improve fertility in those who have irregular ovulatory cycles, determination of the urinary LH by means of Hi-Gonavis appears to be useful, reliable, and economic, both in time and expense.
对25名正常女性进行了两个周期的研究，在第10天至20天对血清促性腺激素、催乳素、雌二醇和孕酮进行放射免疫分析（RIA）。此外，我们每天使用半定量Hi-Gonavis试剂盒检测两次尿液中的促黄体生成素（LH）。最后，每天评估阴道细胞学检查，以确定最佳受孕时间。血清和尿液中的LH在月经周期中期显著增加，促卵泡生成素（FSH）、催乳素、雌二醇和角化指数也显著增加。Hi-Gonavis在排卵日（第0天）前12小时显示出显著增加。这些水平在排卵日达到峰值，并在第0天峰值水平后12小时保持高位。对于接受人工授精的正常女性评估最佳受孕时间，或改善排卵周期不规律女性的生育能力，通过Hi-Gonavis检测尿液LH在时间和费用方面似乎都是有用、可靠且经济的。
