The correlation between LH determination in the urine (Hi-Gonavis) and serum LH, FSH, oestradiol, progesterone, prolactin levels, and vaginal cytology at midcycle.

Radioimmunoassays (RIA) of serum gonadotrophins, prolactin, oestradiol, and progesterone were carried out from day 10 to 20 in 25 normal women for two cycles. In addition, we measured LH in urine twice daily using semiquantitative Hi-Gonavis kits. Finally, vaginal cytology was assessed daily in order to find out the optimum time for conception. LH in serum, as well as in urine, increased significantly at midcycle and so did FSH, prolactin, oestradiol, and the cornification index. Hi-Gonavis showed a significant increase 12 hours prior to the day of ovulation (day 0). The levels reached the peak on the day of ovulation and remained high 12 hours after the peak level on day 0. For the evaluation of the optimum time for conception in normal women who receive artificial insemination, or to improve fertility in those who have irregular ovulatory cycles, determination of the urinary LH by means of Hi-Gonavis appears to be useful, reliable, and economic, both in time and expense.