Leipheimer R E, Condon T P, Curry J J
J Endocrinol. 1984 Mar;100(3):361-5. doi: 10.1677/joe.0.1000361.
Pentobarbitone-blocked pro-oestrous rats were subjected to either limited mating (maximum of 30 mounts), all-night cohabitation with males or stimulation of the vagina and cervix with a glass rod (2 or 5 min) to determine which type of stimulus was most effective in inducing ovulation. All-night cohabitation was the most successful procedure and resulted in 100% ovulation in those rats which mated. Treatment with either phenoxybenzamine, propranolol or pimozide did not interfere with this copulation-induced ovulation whereas methysergide treatment completely blocked copulation-induced ovulation. Administration of atropine resulted in a loss of mating behaviour and these animals therefore did not ovulate. Further experiments provided evidence that administration of atropine also blocked ovulation in response to vaginal stimulation with a glass rod. Pretreatment with methysergide or atropine had no effect upon the percentage of pentobarbitone-blocked, pro-oestrous rats ovulating in response to administration of LH releasing hormone (LHRH). However, those rats given atropine shed significantly fewer ova per rat following LHRH or LH infusion when compared with controls. These results suggest that the synaptic mechanisms responsible for mediating copulation-induced ovulation are different from those mediating steroid-induced ovulation, and that ovarian cholinergic receptors may play a role in ovulation.
有限交配（最多30次爬跨）、与雄性大鼠通宵同居或用玻璃棒刺激阴道和子宫颈（2或5分钟），以确定哪种刺激方式在诱导排卵方面最有效。通宵同居是最成功的方法，那些交配的大鼠排卵率达100%。用酚苄明、普萘洛尔或匹莫齐特处理并不干扰这种交配诱导的排卵，而麦角新碱处理则完全阻断交配诱导的排卵。给予阿托品导致交配行为丧失，因此这些动物不排卵。进一步的实验证明，给予阿托品也能阻断因用玻璃棒刺激阴道而引起的排卵。用麦角新碱或阿托品预处理对戊巴比妥钠麻醉的动情前期大鼠因注射促黄体生成素释放激素（LHRH）而排卵的百分比没有影响。然而，与对照组相比，那些给予阿托品的大鼠在注射LHRH或促黄体生成素后每只大鼠排出的卵子明显较少。这些结果表明，介导交配诱导排卵的突触机制与介导类固醇诱导排卵的机制不同，并且卵巢胆碱能受体可能在排卵中起作用。
