Tyler J L, Gorski R A
Endocrinology. 1980 Jun;106(6):1815-9. doi: 10.1210/endo-106-6-1815.
In two experiments, factors critical to copulation-induced ovulation (CIO) were examined. In Exp I cycling Sprague-Dawley rats were given 25 mg/kg sodium pentobarbital (PB) at 1345 h on proestrus and allowed overnight copulation starting at 1900 (dark onset), 2100, or 2300 h. Only the groups in which copulation began at 1900 or 2100 h displayed ovulation significantly more frequently than PB-treated controls. In Exp II rats received an atrial cannula on diestrus II and 25 mg/kg PB at 1345 h on proestrus. Four blood samples were taken over the course of an hour, starting at 1900 or 2300 h. Controls did not ovulate that night. Rats allowed to copulate for 30 or 60 min starting at 1900 h ovulated and showed elevated plasma LH levels during the period sampled. Copulation at 23 h or cervical probing at 1900 h did not induce LH release or ovulation. There were no differences in the behavior of the males or females during the first 30 min of cohabitation in either experiment; the females were highly receptive. It is concluded that a narrow temporal window exists for CIO, and that temporal changes in induced LH release but not behavior limit CIO.
在两项实验中，对交配诱导排卵（CIO）的关键因素进行了研究。在实验I中，处于发情前期的成年斯普拉格-道利大鼠于13:45被给予25mg/kg戊巴比妥钠（PB），并于19:00（天黑时）、21:00或23:00开始进行夜间交配。只有交配开始于19:00或21:00的组排卵频率显著高于PB处理的对照组。在实验II中，大鼠在动情期II植入心房插管，并于发情前期的13:45给予25mg/kg PB。从19:00或23:00开始，在一小时内采集四份血样。对照组当晚未排卵。从19:00开始交配30或60分钟的大鼠排卵，并在采样期间血浆LH水平升高。23:00交配或19:00宫颈探查未诱导LH释放或排卵。在两个实验中，同居的前30分钟内雄性和雌性的行为均无差异；雌性具有高度接受性。结论是，CIO存在一个狭窄的时间窗，诱导LH释放的时间变化而非行为限制了CIO。
