Pereira M A, Herren-Freund S L, Britt A L, Khoury M M
J Natl Cancer Inst. 1984 Mar;72(3):741-4.
The effect of concurrent administration of phenobarbital on the hepatocarcinogenicity of N-nitrosodiethylamine (diethylnitrosamine; DENA) in rats was investigated by determination of the incidence of gamma-glutamyltransferase (gamma-glutamyltranspeptidase) (GGT)-positive foci and liver tumors. Male outbred Sprague-Dawley rats received either a weekly oral dose of DENA (0.08 mol/kg), phenobarbital sodium (500 ppm) in their drinking water, or DENA and phenobarbital sodium concurrently. After 16 weeks, only the animals treated concurrently with DENA and phenobarbital sodium had GGT-positive foci (3.65 foci/cm2). At 30 weeks, the group treated with DENA and phenobarbital sodium exhibited more foci (23.6 foci/cm2) compared to the group that received only DENA (3.08 foci/cm2). The average size of foci in both of the DENA-treated groups was the same. The tumors in the group that received DENA plus phenobarbital sodium showed a greater incidence of GGT activity compared to the tumors in the DENA group. Under the conditions of this study the incidence of GGT-positive foci did not predict the incidence of hepatocellular carcinomas.
通过测定γ-谷氨酰转移酶（γ-谷氨酰转肽酶）（GGT）阳性病灶和肝肿瘤的发生率，研究了苯巴比妥与N-亚硝基二乙胺（二乙基亚硝胺；DENA）同时给药对大鼠肝癌发生的影响。雄性远交系Sprague-Dawley大鼠每周口服一次DENA（0.08 mol/kg）、饮水中添加苯巴比妥钠（500 ppm）或同时给予DENA和苯巴比妥钠。16周后，只有同时接受DENA和苯巴比妥钠治疗的动物出现GGT阳性病灶（3.65个病灶/cm²）。30周时，与仅接受DENA治疗的组（3.08个病灶/cm²）相比，接受DENA和苯巴比妥钠治疗的组出现了更多病灶（23.6个病灶/cm²）。两个DENA治疗组的病灶平均大小相同。与DENA组的肿瘤相比，接受DENA加苯巴比妥钠治疗组的肿瘤GGT活性发生率更高。在本研究条件下，GGT阳性病灶的发生率并不能预测肝细胞癌的发生率。
