Kiselev V I, Kliucherev S V
Farmakol Toksikol. 1984 Jan-Feb;47(1):26-30.
Study of the effect of medium pH on the mechanism of bradykinin interaction with receptors allowed a suggestion about the presence of a protein component in the composition of bradykinin receptor. Quantitative determination of the pH-dependence of the kinetic parameters of the kininergic reaction indicates the involvement of sulfhydryl groups in the formation of the active center in bradykinin receptor. This finding is supported by experiments with receptor blockade by reagents used for chemical modification of protein. The data obtained also attest to a possible involvement of the imidazole group of histidine in the realization of the myotropic effect of bradykinin.
关于培养基pH对缓激肽与受体相互作用机制影响的研究，提示缓激肽受体组成中存在蛋白质成分。对激肽能反应动力学参数的pH依赖性进行定量测定，表明巯基参与了缓激肽受体活性中心的形成。用于蛋白质化学修饰的试剂对受体进行阻断的实验支持了这一发现。所获得的数据也证明，组氨酸的咪唑基团可能参与了缓激肽肌otropic效应的实现。
