Evensen S A, Ritland S, Elgjo K, Solberg H E, Skrede S
Scand J Gastroenterol. 1984 Jan;19(1):6-13.
Incorporation of 14C-acetate into 3-beta-OH sterols and of 3H-leucine into proteins was examined in liver biopsies from patients with liver disorders. Biopsies obtained from patients in whom no liver disease was found served as controls. Reduced 14C-acetate incorporation into sterols was found in biopsies from patients with chronic active hepatitis and primary biliary cirrhosis. Stimulated incorporation of 3H-leucine into proteins was demonstrated in patients with alcoholic liver cirrhosis and in patients with ulcerative colitis associated with liver disease. No correlation could be established between serum proteins and lipids, respectively, on the one hand, and between incorporation of precursors into proteins and sterols, on the other. 'Incorporation parameters' were also inferior to conventional liver tests when used in the differential diagnosis between different liver disorders by discriminant analysis. Our findings may suggest, however, that hepatic sterol synthesis is frequently decreased in patients with chronic active hepatitis and primary biliary cirrhosis.
对患有肝脏疾病患者的肝活检组织进行了研究，检测了14C - 乙酸盐掺入3-β-羟基固醇以及3H - 亮氨酸掺入蛋白质的情况。从未发现肝脏疾病的患者身上获取的活检组织用作对照。在慢性活动性肝炎和原发性胆汁性肝硬化患者的活检组织中，发现14C - 乙酸盐掺入固醇的量减少。在酒精性肝硬化患者以及伴有肝脏疾病的溃疡性结肠炎患者中，证实了3H - 亮氨酸掺入蛋白质的情况有所增加。血清蛋白和脂质之间，以及前体掺入蛋白质和固醇之间，均未发现相关性。当通过判别分析用于不同肝脏疾病的鉴别诊断时，“掺入参数”也不如传统肝脏检测方法。然而，我们的研究结果可能表明，慢性活动性肝炎和原发性胆汁性肝硬化患者的肝脏固醇合成经常减少。
